The Acid Phosphatase Test (APT) is a presumptive test used to detect the presence of acid phosphatase enzyme in a sample, which can be an indication of the presence of semen. Acid phosphatase is an enzyme that is found in high levels in seminal fluid, and is therefore a useful marker for the presence of semen.
The APT test involves adding a solution to the sample that contains a substrate which is specifically cleaved by acid phosphatase. The reaction produces a yellow color, which can be visually observed or measured with a spectrophotometer. If the sample contains acid phosphatase, the color will change indicating a positive result for semen.
The APT test is relatively simple to perform and can be completed in a relatively short amount of time. However, it is important to note that this test is not specific to semen, as other bodily fluids and some plant materials may also contain acid phosphatase. Therefore, confirmation of the presence of semen using additional tests, such as the Christmas tree stain or microscopic examination for sperm cells, may be necessary to conclusively determine the presence of semen in a sample.